货号:	BI-20892
方法学:	Sandwich ELISA, HRP/TMB, 12x8-well strips
样本类型:	Serum, plasma (EDTA, heparin), cell culture supernatant, urine
标准品范围:	0-10 nmol/l (6 serum based standards)
标准品浓度:	0/0.63/1.25/2.5/5/10 nmol/l
质控品:	1 serum based control
样本量:	10 μl / well
孵育时间:	3 h / 30 min
单位换算:	1 ng/ml = 0.079 nmol/l (MW: 12.7 kDa)

灵敏度：
LOD: 0.05 nmol/l (0 pmol/l + 3 SD)

精密度：　
Intra-assay (n=10) ≤ 2%, Inter-assay (n=5) ≤ 4%

回收率：　
The mean recovery of recombinant proANP (1-98) in human plasma samples (n=4) is 87%.

线性：
Dilution linearity of endogenous proANP (1-98) with assay buffer is:
Mean for 1+1 dilution = 90%
Mean for 1+3 dilution = 87%
Mean for 1+7 dilution = 79%

交叉反应：
The assay cross-reacts with rat or mouse samples.
proANP(1-30) <1%, proANP(31-67) <1%, proANP(79- 98) <1%, alpha ANP( 99-126)<1%, proBNP(8-29)<1%, proBNP(32-57) <1%, proCNP(1-19) <1%, proCNP(30-50) <1%, proCNP(51-97) <1%.

参考范围（健康人群）：
Median (plasma, n=53): 1.45 nmol/l.
推荐实验室建立自己的参考值。
仅供科研使用。